Journal: Immunity

Article Title: The xenobiotic transporter Mdr1 enforces T cell homeostasis in the presence of intestinal bile acids

doi: 10.1016/j.immuni.2017.11.012

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Article Snippet: Rag1 −/− Slc10a2 −/− This paper N/A Oligonucleotides Abcb1a ametrine 5′ CRISPR gRNA: 5′-GCUUCUCAAUGGUCAGUGUGCUGUUUUA GAGCUAGAAAUAGCAAGUUAAAAUAAGG CUAGUCCGUUAUCAACUUGAAAAAGUGG CACCGAGUCGGUGCUUUU-3′ PNA Bio Inc. N/A ametrine Abcb1a 3′ CRISPR gRNA: 5′-GAAAAUACUUAACAUCUUACAUGUUUUA GAGCUAGAAAUAGCAAGUUAAAAUAAGG CUAGUCCGUUAUCAACUUGAAAAAGUGG CACCGAGUCGGUGCUUUU-3′ PNA Bio Inc. N/A Universal 16S rDNA Forward: 5′-ACTCCTACGGGAGGCAGCAGT-3′ Integrated DNA Technologies Ayres et al., 2012 Universal 16S rDNA Reverse: 5′-ATTACCGCGGCTGCTGGC-3′ Integrated DNA Technologies Ayres et al., 2012 Abcb1a ametrine 5′ Genotype Primer: 5′-AGTTTAACGTGTCTGCAGCTGG-3′ Integrated DNA Technologies N/A Abcb1a ametrine 3′ Genotype Primer: 5′-AGCCTGCAGGATCTGTCTG-3′ Integrated DNA Technologies N/A Recombinant DNA Plasmid: LMPd-ametrine Chen et al., 2014 Dr. Matthew Pipkin Plasmid: LMPd-GFP This paper N/A Plasmid: pSTBlue-1 Novagen Cat# 70199 shRNAmir: Cd8a TransOMIC Technologies Cat# TLMSU1400-12525 shRNAmir: Abcb1a TransOMIC Technologies Cat# TLMSU1400-18671 shRNAmir: Abcb1b TransOMIC Technologies Cat# TLMSU1400-18669 pHaMDRwt Pastan et al., 1988 Addgene Plasmid #10957 LMPd.hMDR1-ametrine (containing wild-type human MDR1) (NCBI reference sequence {"type":"entrez-nucleotide","attrs":{"text":"NM_001348945.1","term_id":"1149123046"}} NM_001348945.1 ) This paper N/A LMPd. hMDR1-ametrine (containing transport-deficient human MDR1; Y401A, Y1044A) α This paper Kim et al., 2006 Software and Algorithms Graphpad Prism 7 GraphPad Software http://www.graphpad.com FlowJo (Version 9.9.4) TreeStar, Inc. http://www.flowjo.com GenePattern (Version 3.9.10) Broad Institute https://genepattern.broadinstitute.org nSolver Analysis Software (Version 1.1) Nanostring https://www.nanostring.com Other NanoString nCounter Sundrud_03 chip This paper https://www.nanostring.com NanoString nCounter Sundrud_04 chip This paper https://www.nanostring.com Open in a separate window

Techniques: Blocking Assay, Purification, Recombinant, Staining, Gene Expression, Magnetic Beads, Cell Isolation, SYBR Green Assay, Mutagenesis, Detection Assay, Fluorescence, Generated, CRISPR, Plasmid Preparation, Sequencing, Software